Loading Plate Data in FLIMfit

Setup calibration data

• Determine IRF shift

  • Load reference data
  • Set background, rep rate
  • Load library IRF
  • Adjust ‘IRF shift’ until the edge of the IRF sits roughly at the rising edge
  • Fit mono exponential model with free IRF shift
  • Check quality of fit
  • Note t0 value. This value should be subtracted from the IRF shift used

• Create time varying background

  • Load media only well
  • Load camera background
  • Select central region
  • Select Tools>Export SV TVB...

Loading data

• Load data
• Load plate metadata
• Load camera background images
• Set laser repetition rate
• Set gate maximum to camera saturation value
• Load IRF and set IRF shift from reference measurement
• Load time varying background
• Save as raw dataset